Cy2-NHS，Cy2-NHS ester，NHS ester-Cy2，186205-33-4

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Cy2-NHS酯(氰基2-N-羟基琥珀酰亚胺酯)是一种荧光染料，在生物研究和成像应用中常用于标记蛋白质、肽和其他生物分子。

Cy2-NHS酯中的NHS酯官能团允许染料共价连接到生物分子上的伯胺(-NH2)上。该反应通过在NHS酯基和氨基之间形成酰胺键而发生。这种标记方法由于其稳定性和有效性而具有高度选择性并被广泛使用。

一旦连接到生物分子上，Cy2-NHS酯在大约495纳米的波长下表现出强烈的荧光发射，这属于绿色光谱。它可以使用适当的激发源进行可视化，例如激光或特定的滤波器。

Cy2-NHS酯适用于各种应用，包括免疫荧光、流式细胞术、荧光显微镜和用于检测和定量的蛋白质标记。其明亮和耐光的荧光特性使其成为多色标记实验的热门选择。

需要注意的是，Cy2-NHS酯的正确处理和储存对于保持其稳定性和有效性是必要的。遵循制造商的使用说明和指南对于染色和成像结果的成功至关重要。

Cy2-NHS ester (Cyanine 2-N-hydroxysuccinimide ester) is a fluorescent dye commonly used for labeling proteins, peptides, and other biomolecules in biological research and imaging applications.

The NHS ester functional group in Cy2-NHS ester allows for the covalent attachment of the dye to primary amines (-NH2) on biomolecules. The reaction occurs through the formation of an amide bond between the NHS ester group and the amino group. This labeling method is highly selective and widely used due to its stability and efficiency.

Once attached to the biomolecule, Cy2-NHS ester exhibits strong fluorescence emission at a wavelength of approximately 495 nanometers, which falls within the green spectrum. It can be visualized using appropriate excitation sources, such as laser or specific filters.

Cy2-NHS ester is suitable for various applications, including immunofluorescence, flow cytometry, fluorescence microscopy, and protein labeling for detection and quantification. Its bright and photostable fluorescence properties make it a popular choice for multicolor labeling experiments.

It is important to note that proper handling and storage of Cy2-NHS ester are necessary to maintain its stability and effectiveness. Following the manufacturer"s instructions and guidelines for use is crucial for successful staining and imaging results.

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